About TrypsnetDB2

Overview

TrypsnetDB2 is a database of predicted protein–protein interactions (PPIs) across 20 trypanosomatid organisms. Interactions were transferred from four experimentally validated PPI datasets using orthology group (OG) mappings at two levels of resolution (OG6 and OG7).

Source datasets
DatasetOrganismMethod
T.brucei_2016_GazestaniTrypanosoma brucei brucei TREU927Co-fractionation MS
T.brucei_2017_CrozierTrypanosoma brucei brucei TREU927Co-fractionation MS
L.donovani_2025_AzimiLeishmania donovani BPK282A1Co-fractionation MS
T.cruzi_2025_AzimiTrypanosoma cruzi Sylvio X10/1-2012Co-fractionation MS
Transfer methodology
  1. Source interactions were mapped to ortholog group identifiers at two resolution levels: OG6 and OG7 (OrthoFinder outputs).
  2. For each source interaction (protein A – protein B), all genes in the target organism sharing the same OG as A were paired with all genes sharing the same OG as B.
  3. A paralog cap of 10 was applied: interactions were not transferred when either OG contained more than 10 members in the target organism, preventing spurious expansion from large repetitive gene families.
  4. Each transferred interaction is scored by:
    • OG support: OG6 only / OG7 only / both
    • Experimental count: number of the 4 source datasets that independently support the interaction (1–4)
  5. Paralog pooling: when a source gene belongs to an OG with multiple members in the source organism, all members' interactions are pooled. Cases where this occurred are flagged in the source_paralogs_raw field.
Scoring guide
FieldMeaningHigher = ?
Experimental count Number of the 4 source datasets supporting the interaction (1–4) Higher confidence
Both OG6 & OG7 Both ortholog group versions support the transfer More robust orthology
⚠ Paralog flag Source or target had multiple paralogs in the OG; interaction was inferred by pooling Lower specificity
Data download

Full CSV downloads are available from each gene and organism page. For bulk data access, contact the authors.