Database Statistics

An overview of the orthology-transferred PPI data across all 20 trypanosomatid organisms.

1,535,794
Transferred interactions
Across all 20 organisms
176,431
Genes / proteins
Unique gene identifiers
20
Target organisms
Across Trypanosomatida
38,851
Source PPI edges
From 4 experimental datasets
Advance over TrypsnetDB (v1): TrypsnetDB v1 covered 15 organisms with ~101,000 interactions. TrypsnetDB2 expands this to 20 organisms with 1,535,794 transferred interactions and introduces dual-resolution orthology (OG6 + OG7), a paralog cap, and two-layer evidence scoring.
Interaction support breakdown
Ortholog group support
OG6 only 134,913
OG7 only 889,249
Both OG6 & OG7 726,725
Orthology evidence score (0–3)
0 source organisms 36,660
1 source organism 1,711,683
2 source organisms 33,941
3 source organisms 5,264
Score = number of main source organisms (T.b / T.c / L.d) whose PPIs support the interaction via orthology. Max 3 for non-source organisms; max 2 for the three source organisms themselves.
By genus
Trypanosoma 804,699
Leishmania 786,455
Leptomonas 101,939
Crithidia 94,455
Direct experimental evidence (Co-frac/MS)
Direct experimental evidence (primary_evidence > 0) is available only for the three source organisms — T. brucei TREU927, T. cruzi SYLVIO, and L. donovani BPK282A1 — where exact co-fractionation MS gene pairs are stored without any ortholog mapping. All 17 other organisms rely entirely on orthology inference (secondary evidence only).
primary = 0 1,748,911 interactions primary = 1 38,423 interactions primary = 2 214 interactions
Source datasets
T.brucei_2016_Gazestani
Trypanosoma brucei brucei TREU927
5,134 edges
T.brucei_2017_Crozier
Trypanosoma brucei brucei TREU927
1,650 edges
L.donovani_2025_Azimi
Leishmania donovani BPK282A1
15,991 edges
T.cruzi_2025_Azimi
Trypanosoma cruzi Sylvio X10/1-2012
16,076 edges
Interactions per organism
Organism Interactions OG6 OG7 Both OG Max primary Max secondary
Trypanosoma Trypanosoma cruzi Dm28c 2018 127,446 70,687 111,759 55,000 0 3
Leptomonas Leptomonas pyrrhocoris H10 101,939 51,836 92,412 42,309 0 3
Trypanosoma Trypanosoma congolense IL3000 100,998 51,031 91,485 41,518 0 3
Leishmania Leishmania braziliensis MHOM/BR/75/M2904 94,498 47,993 87,932 41,427 0 3
Crithidia Crithidia fasciculata strain Cf-Cl 94,455 45,850 87,482 38,877 0 3
Leishmania Leishmania tropica L590 94,281 46,205 87,793 39,717 0 3
Trypanosoma Trypanosoma brucei brucei TREU927 93,466 35,476 81,375 29,253 2 2
Leishmania Leishmania major strain Friedlin 93,123 46,820 87,191 40,888 0 3
Leishmania Leishmania mexicana MHOM/GT/2001/U1103 93,048 47,027 86,635 40,614 0 3
Trypanosoma Trypanosoma cruzi Y C6 92,130 47,682 83,410 38,962 0 3
Trypanosoma Trypanosoma rangeli SC58 91,819 38,846 87,993 35,021 0 3
Leishmania Leishmania aethiopica L147 91,219 45,406 84,468 38,655 0 3
Leishmania Leishmania infantum JPCM5 91,177 46,662 84,916 40,401 0 3
Leishmania Leishmania panamensis MHOM/COL/81/L13 89,435 44,725 82,717 38,007 0 3
Trypanosoma Trypanosoma evansi strain STIB 805 82,136 38,113 77,457 33,434 0 3
Trypanosoma Trypanosoma vivax Y486 81,010 40,735 74,465 34,190 0 3
Trypanosoma Trypanosoma cruzi CL Brener Esmeraldo-like 78,734 38,318 72,330 31,914 0 3
Leishmania Leishmania amazonensis MHOM/BR/71973/M2269 78,009 36,822 72,458 31,271 0 3
Leishmania Leishmania donovani BPK282A1 61,665 25,027 42,701 21,394 1 2
Trypanosoma Trypanosoma cruzi Sylvio X10/1-2012 56,960 16,377 38,995 13,873 1 2